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KMID : 0363320050260010093
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Journal of Korean Oriental Internal Medicine
2005 Volume.26 No. 1 p.93 ~ p.106
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The Effect of Chungganhaeju-tang on TGF-{beta}1-induced Hepatic Fibrosis
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Lee Ji-Hyun
Lee Jang-Hoon
Kim Young-Chul
Woo Hong-Jung
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Abstract
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Objectives : The aim of this study is to characterize the effect of Chungganhaeju-tang on TGF-{beta}l-induced hepatic fibrosis.
Materials and Methods : mRNA and protein expression levels of TGF-{beta}l in Chungganhaeju-tang treated HepG2 cells were compared to untreated cells using quantitative RT-PCR and ELISA assay, respectively. mRNA expression levels of the TGF-{beta}l signaling pathway genes (T{beta}R-I,;T{beta}R-II,;Smad2,;Smad3,;Smad4,;and;PAI-1) and fibrosis-associated genes (CTGF, fibronectin, and collagen type l{alpha}) were evaluated by quantitative RT-PCR. The effect of Chungganhaeju-tang on cell proliferation of T3891 human fibroblast was evaluated using [3H]Thymidine Incorporation Assay.
Results : Inhibition of TGF-{beta}l mRNA expression and protein production was observed with treatment of Chungganhaeju-tang and seen to be dose and time dependent. Whereas TGF-{beta}l-mediated induction of PAI-1 was suppressed with treatment of Chungganhaeju-tang, expression of the TGF-{beta}l signaling pathway genes such as T{beta}R-I, T{beta}R-II, Smad2, Smad3, and Smad4 was not affected. With treatment of Chungganhaeju-tang, inhibition of TGF-{beta}l-induced cell proliferation of T3891 human fibroblast was observed, as well as abrogation of TGF-{beta}l-mediated transcriptional up-regulation of CTGF, fibronectin, and collagen type I{alpha}.
Conclusion : This study strongly suggests that the liver cirrhosis-suppressive activity of Chungganhaeju-tang may be derived at least in part from its inhibitory effect on TGF-{beta}l functions, such as blockade of TGF-{beta}l stimulation of fibroblast cell proliferation and fibrosis-related gene expression as well as expression of TGF-{beta}l itself.
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KEYWORD
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Chungganhaeju-tang, hepatic fibrosis, TGF-{beta}1
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